Cattle EG-VEGF(Endocrine Gland Derived Vascular Endothelial Growth Factor) ELISA Kit

Artikelnummer: EBT-ELK5971
Artikelname: Cattle EG-VEGF(Endocrine Gland Derived Vascular Endothelial Growth Factor) ELISA Kit
Artikelnummer: EBT-ELK5971
Hersteller Artikelnummer: ELK5971
Alternativnummer: EBT-ELK5971-96, EBT-ELK5971-48, EBT-ELK5971-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Bovine
Alternative Synonym: PK1, EGVEGF, PRK1, PROK1, Prokineticin 1, Black Mamba Toxin-Related Protein, Mambakine
Konzentration: 1000 pg/mL
Detektionsbereich: 15.63-1000 pg/mL
Sensitivitaet: 6.3 pg/mL
Proben: Serum, plasma and other biological fluids.
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Cytokine,Tumor immunity,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle EG-VEGF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle EG-VEGF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle EG-VEGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Cattle EG-VEGF in the samples is then determined by comparing the OD of the samples to the standard curve
ELK5971