Rat VEGFR1(Vascular Endothelial Growth Factor Receptor 1) ELISA Kit

Artikelnummer: EBT-ELK6010
Artikelname: Rat VEGFR1(Vascular Endothelial Growth Factor Receptor 1) ELISA Kit
Artikelnummer: EBT-ELK6010
Hersteller Artikelnummer: ELK6010
Alternativnummer: EBT-ELK6010-96, EBT-ELK6010-48, EBT-ELK6010-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Rat
Alternative Synonym: Flt1, Fms-Related Tyrosine Kinase 1, Vascular Permeability Factor Receptor, Fms-like tyrosine kinase 1, Tyrosine-protein kinase receptor FLT
Konzentration: 10 ng/mL
Detektionsbereich: 0.16-10 ng/mL
Sensitivitaet: 0.059 ng/mL
UniProt: P53767
Proben: Serum, plasma, tissue homogenates and other biological fluids
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Signal transduction,Tumor immunity,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat VEGFR1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat VEGFR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat VEGFR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rat VEGFR1 in the samples is then determined by comparing the OD of the samples to the standard curve
ELK6010