Mouse MYO5A(Myosin VA) Microsample ELISA Kit

Artikelnummer: EBT-ELK6050MS
Artikelname: Mouse MYO5A(Myosin VA) Microsample ELISA Kit
Artikelnummer: EBT-ELK6050MS
Hersteller Artikelnummer: ELK6050MS
Alternativnummer: EBT-ELK6050MS-96, EBT-ELK6050MS-48, EBT-ELK6050MS-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Mouse
Alternative Synonym: GS1, MYH12, MYO5, MYOXIN, MYR12, myosin V, myosin Va, Myosin,Heavy Polypeptide Kinase, Myosin Heavy Chain 12, Myoxin, Myosin V, Dilute myosin heavy chain, non-muscle
Konzentration: 10 ng/mL
Detektionsbereich: 0.16-10 ng/mL
Sensitivitaet: 0.046 ng/mL
UniProt: Q99104
Proben: Tissue homogenates and other biological fluids.
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Enzyme & Kinase,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse MYO5A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MYO5A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse MYO5A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Mouse MYO5A in the samples is then determined by comparing the OD of the samples to the standard curve
ELK6050MS