Human Anti-GAD(Anti-Glutamic Acid Decarboxylase Antibody) ELISA Kit

Artikelnummer: EBT-ELK7911
Artikelname: Human Anti-GAD(Anti-Glutamic Acid Decarboxylase Antibody) ELISA Kit
Artikelnummer: EBT-ELK7911
Hersteller Artikelnummer: ELK7911
Alternativnummer: EBT-ELK7911-96,EBT-ELK7911-48,EBT-ELK7911-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Human
Alternative Synonym: Glutamate Decarboxylase
Konzentration: 100 ng/mL
Detektionsbereich: 1.57-100 ng/mL
Sensitivitaet: 0.59 ng/mL
Proben: Serum, plasma and other biological fluids
Anwendungsbeschreibung: Assay Type: Competitive Inhibition. Assay length: 2.5h. Research Area: Enzyme & Kinase,Endocrinology,Neuro science,Autoimmunity,. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Anti-GAD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-GAD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human Anti-GAD in the samples is then determined by comparing the OD of the samples to the standard curve