Rat TLR1(Toll Like Receptor 1) ELISA Kit

Artikelnummer: EBT-ELK8390
Artikelname: Rat TLR1(Toll Like Receptor 1) ELISA Kit
Artikelnummer: EBT-ELK8390
Hersteller Artikelnummer: ELK8390
Alternativnummer: EBT-ELK8390-96,EBT-ELK8390-48,EBT-ELK8390-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Rat
Alternative Synonym: CD281, TIL, Rsc786, Toll/interleukin-1 receptor-like protein
Konzentration: 20 ng/mL
Detektionsbereich: 0.32-20 ng/mL
Sensitivitaet: 0.111 ng/mL
Proben: Serum, plasma, tissue homogenates and other biological fluids.
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Signal transduction,Tumor immunity,Infection immunity,Immune molecule,Pulmonology,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TLR1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TLR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TLR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rat TLR1 in the samples is then determined by comparing the OD of the samples to the standard curve