Human 8-iso prostaglandin F2alpha (8-iso-PGF2a) ELISA Kit

Produktübersicht

• Artikelname: Human 8-iso prostaglandin F2alpha (8-iso-PGF2a) ELISA Kit
• Artikelnummer: EBT-ELK8402
• Hersteller Artikelnummer: ELK8402
• Alternativnummer: EBT-ELK8402-96,EBT-ELK8402-48,EBT-ELK8402-96X5
• Hersteller: ELK Biotechnology
• Kategorie: Kits/Assays
• Spezies Reaktivität: Human
• Alternative Synonym: 8-isoprostane, 8-iso-PGF2alpha

Produkteigenschaften

• Konzentration: 2000 pg/mL
• Detektionsbereich: 31.25-2000 pg/mL
• Sensitivitaet: 8 pg/mL
• Proben: serum, plasma and other biological fluids

Anwendungsbeschreibung

• Anwendungsbeschreibung: Assay Type: Competitive Inhibition. Assay length: 2h. Research Area: Endocrinology,Pulmonology,Hepatology,Hormone metabolism,. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human 8-iso-PGF2a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 8-iso-PGF2a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human 8-iso-PGF2a in the samples is then determined by comparing the OD of the samples to the standard curve