Horse sCD14(soluble cluster of differentiation 14) ELISA Kit

Artikelnummer: EBT-ELK9675
Artikelname: Horse sCD14(soluble cluster of differentiation 14) ELISA Kit
Artikelnummer: EBT-ELK9675
Hersteller Artikelnummer: ELK9675
Alternativnummer: EBT-ELK9675-96, EBT-ELK9675-48, EBT-ELK9675-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Equine
Alternative Synonym: CD14 Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein, CD antigen CD14, soluble cluster of differentiation 14
Konzentration: 20 ng/mL
Detektionsbereich: 0.32-20 ng/mL
Sensitivitaet: 0.238 ng/mL
Proben: serum, plasma, cell lysates and other biological fluids
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: CD & Adhesion molecule,Tumor immunity,Infection immunity,Immune molecule,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Horse sCD14. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse sCD14. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Horse sCD14, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Horse sCD14 in the samples is then determined by comparing the OD of the samples to the standard curve
ELK9675