Horse HMGB-2(HighMobility Group Protein B2) ELISA Kit

Produktübersicht

• Artikelname: Horse HMGB-2(HighMobility Group Protein B2) ELISA Kit
• Artikelnummer: EBT-ELK9976
• Hersteller Artikelnummer: ELK9976
• Alternativnummer: EBT-ELK9976-96, EBT-ELK9976-48, EBT-ELK9976-96X5
• Hersteller: ELK Biotechnology
• Kategorie: Kits/Assays
• Spezies Reaktivität: Equine
• Alternative Synonym: High mobility group protein B2

Produkteigenschaften

• Konzentration: 10 ng/mL
• Detektionsbereich: 0.16-10 ng/mL
• Sensitivitaet: 0.062 ng/mL
• Proben: Serum, plasma and other biological fluids

Anwendungsbeschreibung

• Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Infection immunity,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Horse HMGB-2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse HMGB-2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Horse HMGB-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Horse HMGB-2 in the samples is then determined by comparing the OD of the samples to the standard curve

Bilder

ELK9976