GSK3alpha/beta (phospho Tyr279/216) rabbit pAb, Rabbit, Polyclonal

Produktübersicht

• Artikelname: GSK3alpha/beta (phospho Tyr279/216) rabbit pAb, Rabbit, Polyclonal
• Artikelnummer: EBT-ES1321
• Hersteller Artikelnummer: ES1321
• Alternativnummer: EBT-ES1321-50, EBT-ES1321-100
• Hersteller: ELK Biotechnology
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, IHC, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: The antiserum was produced against synthesized peptide derived from human GSK3 alpha/beta around the phosphorylation site of Tyr279/216. AA range:246-295
• Alternative Synonym: GSK3A, Glycogen synthase kinase-3 alpha, GSK-3 alpha, Serine/threonine-protein kinase GSK3A, GSK3B, Glycogen synthase kinase-3 beta, GSK-3 beta, Serine/threonine-protein kinase GSK3B

Produktbeschreibung

glycogen synthase kinase 3 alpha(GSK3A) Homo sapiens This gene encodes a multifunctional Ser/Thr protein kinase that is implicated in the control of several regulatory proteins including glycogen synthase, and transcription factors, such as JUN. It also plays a role in the WNT and PI3K signaling pathways, as well as regulates the production of beta-amyloid peptides associated with Alzheimers disease. [provided by RefSeq, Oct 2011],

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1 mg/ml
• Molekulargewicht: 51,46kD
• NCBI: 2931
• UniProt: P49840

Anwendungsbeschreibung

• Application Verdünnung: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.

Bilder

Immunofluorescence analysis of Hela cell. 1,GSK3alpha/beta (phospho Tyr279/216) Polyclonal Antibody(green) was diluted at 1:200(4 overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog:RS3211 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min.

Immunofluorescence analysis of mouse-liver tissue. 1,GSK3alpha/beta (phospho Tyr279/216) Polyclonal Antibody(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of mouse-liver tissue. 1,GSK3alpha/beta (phospho Tyr279/216) Polyclonal Antibody(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Western Blot analysis of various cells using primary antibody diluted at 1:1000(4C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25C, 1 hour). Cell lysate was extracted by Minute(TM) Plasma Membrane Protein Isolation and Cell Fractionation Kit(SM-005, Inventbiotech,MN,USA).