This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. [provided by RefSeq, Jul 2008],
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
Western Blot analysis of various cells using Phospho-IKKalpha (T23) Polyclonal Antibody diluted at 1:1000
Western Blot analysis of KB cells using Phospho-IKKalpha (T23) Polyclonal Antibody diluted at 1:1000
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using IKK-alpha (Phospho-Thr23) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from MDA-MB-435 cells treated with EGF, using IKK-alpha (Phospho-Thr23) Antibody. The lane on the left is blocked with the phospho peptide.
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