The antiserum was produced against synthesized peptide derived from human IKK-alpha/beta around the phosphorylation site of Ser180/181. AA range:151-200
This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. [provided by RefSeq, Jul 2008],
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Western Blot analysis of various cells using Phospho-IKKalpha/beta (S180/181) Polyclonal Antibody diluted at 1:1000
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using IKK-alpha/beta (Phospho-Ser180/181) Antibody
Immunohistochemistry analysis of paraffin-embedded human heart, using IKK-alpha/beta (Phospho-Ser180/181) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from HepG2 cells treated with TNF 20ng/ml 5, using IKK-alpha/beta (Phospho-Ser180/181) Antibody. The lane on the right is blocked with the phospho peptide.
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