The antiserum was produced against synthesized peptide derived from human IRS-1 around the phosphorylation site of Ser307. AA range:274-323
Alternative Synonym:
IRS1, Insulin receptor substrate 1, IRS-1
This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. [provided by RefSeq, Nov 2009],
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Western Blot analysis of various cells using Phospho-IRS-1 (S307) Polyclonal Antibody diluted at 1:1000
Western Blot analysis of VEC cells using Phospho-IRS-1 (S307) Polyclonal Antibody diluted at 1:1000
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using IRS-1 (Phospho-Ser307) Antibody
Immunofluorescence analysis of COS7 cells, using IRS-1 (Phospho-Ser307) Antibody. The picture on the right is blocked with the phospho peptide.
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