NFkappaB-p105 (phospho Ser932) rabbit pAb, Rabbit, Polyclonal

Produktübersicht

• Artikelname: NFkappaB-p105 (phospho Ser932) rabbit pAb, Rabbit, Polyclonal
• Artikelnummer: EBT-ES1367
• Hersteller Artikelnummer: ES1367
• Alternativnummer: EBT-ES1367-50, EBT-ES1367-100
• Hersteller: ELK Biotechnology
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, IHC, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: The antiserum was produced against synthesized peptide derived from human NF-kappaB p105/p50 around the phosphorylation site of Ser932. AA range:899-948
• Alternative Synonym: NFKB1, Nuclear factor NF-kappa-B p105 subunit, DNA-binding factor KBF1, EBP-1, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1

Produktbeschreibung

nuclear factor kappa B subunit 1(NFKB1) Homo sapiens This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Alternative splicing results in multiple transcript variants encoding different isof

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1 mg/ml
• Molekulargewicht: 105kD
• NCBI: 4790
• UniProt: P19838

Anwendungsbeschreibung

• Application Verdünnung: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.

Bilder

Western Blot analysis of various cells using Phospho-NFkappaB-p105 (S932) Polyclonal Antibody

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using NF-kappaB p105/p50 (Phospho-Ser932) Antibody

Western blot analysis of lysates from HeLa cells treated with CA+TNF 20ng/ml 10, using NF-kappaB p105/p50 (Phospho-Ser932) Antibody. The lane on the left is blocked with the phospho peptide.

Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4 overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).