NFkappaB-p65 (phospho Ser529) rabbit pAb, Rabbit, Polyclonal

Produktübersicht

• Artikelname: NFkappaB-p65 (phospho Ser529) rabbit pAb, Rabbit, Polyclonal
• Artikelnummer: EBT-ES1371
• Hersteller Artikelnummer: ES1371
• Alternativnummer: EBT-ES1371-50, EBT-ES1371-100
• Hersteller: ELK Biotechnology
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, IHC, WB
• Spezies Reaktivität: Human, Mouse
• Immunogen: The antiserum was produced against synthesized peptide derived from human NF-kappaB p65 around the phosphorylation site of Ser529. AA range:496-545
• Alternative Synonym: RELA, NFKB3, Transcription factor p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3

Produktbeschreibung

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011],

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1 mg/ml
• Molekulargewicht: 65kD
• NCBI: 5970
• UniProt: Q04206

Anwendungsbeschreibung

• Application Verdünnung: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.

Bilder

Western Blot analysis of various cells using Phospho-NFkappaB-p65 (S529) Polyclonal Antibody diluted at 1:2000

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using NF-kappaB p65 (Phospho-Ser529) Antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using NF-kappaB p65 (Phospho-Ser529) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from HeLa and Jurkat cells, using NF-kappaB p65 (Phospho-Ser529) Antibody. The lane on the right is blocked with the phospho peptide.