p38 (phospho Thr180) rabbit pAb, Rabbit, Polyclonal

Produktübersicht

• Artikelname: p38 (phospho Thr180) rabbit pAb, Rabbit, Polyclonal
• Artikelnummer: EBT-ES1379
• Hersteller Artikelnummer: ES1379
• Alternativnummer: EBT-ES1379-50, EBT-ES1379-100
• Hersteller: ELK Biotechnology
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, IHC, WB
• Spezies Reaktivität: Human, Monkey, Mouse, Rat
• Immunogen: The antiserum was produced against synthesized peptide derived from human p38 MAPK around the phosphorylation site of Thr180. AA range:147-196
• Alternative Synonym: MAPK14, CSBP, CSBP1, CSBP2, CSPB1, MXI2, SAPK2A, Mitogen-activated protein kinase 14, MAP kinase 14, MAPK 14, Cytokine suppressive anti-inflammatory drug-binding protein, CSAID-binding protein, CSBP, MAP kinase MXI2, MAX-interacting protein

Produktbeschreibung

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding d

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1 mg/ml
• Molekulargewicht: 38kD
• NCBI: 1432
• UniProt: Q16539

Anwendungsbeschreibung

• Application Verdünnung: WB 1:500-2000, IF 1:50-300, IHC 1:50-300 IHC 1:50-300

Bilder

Immunofluorescence analysis of Hela cell. 1,p38 (phospho Thr180) Polyclonal Antibody(green) was diluted at 1:200(4 overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog:RS3211 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min.

Immunofluorescence analysis of rat-lung tissue. 1,p38 (phospho Thr180) Polyclonal Antibody(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of rat-lung tissue. 1,p38 (phospho Thr180) Polyclonal Antibody(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,p38 (phospho Thr180) Polyclonal Antibody was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.