p27 (phospho Thr187) rabbit pAb, Rabbit, Polyclonal

Produktübersicht

• Artikelname: p27 (phospho Thr187) rabbit pAb, Rabbit, Polyclonal
• Artikelnummer: EBT-ES1418
• Hersteller Artikelnummer: ES1418
• Alternativnummer: EBT-ES1418-50, EBT-ES1418-100
• Hersteller: ELK Biotechnology
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, IHC, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: The antiserum was produced against synthesized peptide derived from human p27 Kip1 around the phosphorylation site of Thr187. AA range:149-198
• Alternative Synonym: CDKN1B, KIP1, Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1

Produktbeschreibung

This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4). [provided by RefSeq, Apr 2014],

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1 mg/ml
• NCBI: 1027
• UniProt: P46527

Anwendungsbeschreibung

• Application Verdünnung: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.

Bilder

Western Blot analysis of various cells using Phospho-p27 (T187) Polyclonal Antibody

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using p27 Kip1 (Phospho-Thr187) Antibody

Western blot analysis of lysates from HeLa cells treated with EGF or IFN-alpha, using p27 Kip1 (Phospho-Thr187) Antibody. The lane on the left is blocked with the phospho peptide.

Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4 overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).