The antiserum was produced against synthesized peptide derived from human eNOS around the phosphorylation site of Ser1176. AA range:1144-1193
Alternative Synonym:
NOS3, Nitric oxide synthase, endothelial, Constitutive NOS, cNOS, EC-NOS, Endothelial NOS, eNOS, NOS type III, NOSIII
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009],
Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Immunofluorescence analysis of A549. 1,primary Antibody(red) was diluted at 1:200(4C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min.
Immunofluorescence analysis of Hela cell. 1,NOS3 (phospho Ser1177) Polyclonal Antibody(green) was diluted at 1:200(4 overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog:RS3211 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min.
Western Blot analysis of various cells using Phospho-NOS3 (S1177) Polyclonal Antibody diluted at 1:1000
Western Blot analysis of HuvEc cells using Phospho-NOS3 (S1177) Polyclonal Antibody diluted at 1:1000
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