Shc (phospho Tyr427) rabbit pAb, Rabbit, Polyclonal

Produktübersicht

• Artikelname: Shc (phospho Tyr427) rabbit pAb, Rabbit, Polyclonal
• Artikelnummer: EBT-ES1454
• Hersteller Artikelnummer: ES1454
• Alternativnummer: EBT-ES1454-50, EBT-ES1454-100
• Hersteller: ELK Biotechnology
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, IHC, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: The antiserum was produced against synthesized peptide derived from human Shc around the phosphorylation site of Tyr427. AA range:393-442
• Alternative Synonym: SHC1, SHC, SHCA, SHC-transforming protein 1, SHC-transforming protein 3, SHC-transforming protein A, Src homology 2 domain-containing-transforming protein C1, SH2 domain protein C1

Produktbeschreibung

This gene encodes three main isoforms that differ in activities and subcellular location. While all three are adapter proteins in signal transduction pathways, the longest (p66Shc) may be involved in regulating life span and the effects of reactive oxygen species. The other two isoforms, p52Shc and p46Shc, link activated receptor tyrosine kinases to the Ras pathway by recruitment of the GRB2/SOS complex. p66Shc is not involved in Ras activation. Unlike the other two isoforms, p46Shc is targeted to the mitochondrial matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011],

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1 mg/ml
• Molekulargewicht: 66(p66 isoform), 52(p52 isoform), 46(p46 isoform)kD
• NCBI: 6464
• UniProt: P29353

Anwendungsbeschreibung

• Application Verdünnung: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.

Bilder

Western Blot analysis of various cells using Phospho-Shc (Y427) Polyclonal Antibody diluted at 1:500

Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100(4 overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunohistochemistry analysis of paraffin-embedded human brain, using Shc (Phospho-Tyr427) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from 293 cells treated with EGF 200ng/ml 5, using Shc (Phospho-Tyr427) Antibody. The lane on the right is blocked with the phospho peptide.