The antiserum was produced against synthesized peptide derived from human 14-3-3 zeta around the phosphorylation site of Ser58. AA range:24-73
Alternative Synonym:
YWHAZ, 14-3-3 protein zeta/delta, Protein kinase C inhibitor protein 1, KCIP-1
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5 UTR but that encode the same protein have been identified for this gene. [provided by RefSeq, Oct 2008],
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Western Blot analysis of various cells using Phospho-14-3-3 zeta (S58) Polyclonal Antibody diluted at 1:2000
Western Blot analysis of Jurkat cells using Phospho-14-3-3 zeta (S58) Polyclonal Antibody diluted at 1:2000
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using 14-3-3 zeta (Phospho-Ser58) Antibody
Immunofluorescence analysis of HeLa cells treated with PMA 125ng/ml 30, using 14-3-3 zeta (Phospho-Ser58) Antibody. The picture on the right is blocked with the phospho peptide.
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