The antiserum was produced against synthesized peptide derived from human 14-3-3 thet/tau. AA range:196-245
Alternative Synonym:
YWHAQ, 14-3-3 protein theta, 14-3-3 protein T-cell, 14-3-3 protein tau, Protein HS1
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5 UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease. [provided by RefSeq, Jul 2008],
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Western Blot analysis of various cells using 14-3-3 theta/tau Polyclonal Antibody diluted at 1:500
Immunofluorescence analysis of HeLa cells, using 14-3-3 thet/tau Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from HeLa cells, using 14-3-3 thet/tau Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4 overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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