Actinin-alpha2/3 rabbit pAb, Rabbit, Polyclonal

Produktübersicht

• Artikelname: Actinin-alpha2/3 rabbit pAb, Rabbit, Polyclonal
• Artikelnummer: EBT-ES1594
• Hersteller Artikelnummer: ES1594
• Alternativnummer: EBT-ES1594-50, EBT-ES1594-100
• Hersteller: ELK Biotechnology
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, IHC, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: The antiserum was produced against synthesized peptide derived from human Actinin alpha-2/3. AA range:31-80
• Alternative Synonym: ACTN2, Alpha-actinin-2, Alpha-actinin skeletal muscle isoform 2, F-actin cross-linking protein, ACTN3, Alpha-actinin-3, Alpha-actinin skeletal muscle isoform 3, F-actin cross-linking protein

Produktbeschreibung

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2013],

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1 mg/ml
• Molekulargewicht: 103kD
• NCBI: 88
• UniProt: P35609

Anwendungsbeschreibung

• Application Verdünnung: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.

Bilder

Western Blot analysis of various cells using Actinin-alpha2/3 Polyclonal Antibody

Western Blot analysis of HuvEc cells using Actinin-alpha2/3 Polyclonal Antibody

Immunofluorescence analysis of HeLa cells, using Actinin alpha-2/3 Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue, using Actinin alpha-2/3 Antibody. The picture on the right is blocked with the synthesized peptide.