The antiserum was produced against synthesized peptide derived from human AKR1CL2. AA range:141-190
Alternative Synonym:
AKR1E2, AKR1CL2, AKRDC1, 1, 5-anhydro-D-fructose reductase, AF reductase, Aldo-keto reductase family 1 member C-like protein 2, Aldo-keto reductase family 1 member E2, LoopADR, Testis-specific protein, hTSP
The protein encoded by this gene is a member of the aldo-keto reductase superfamily. Members in this family are characterized by their structure (evolutionarily highly conserved TIM barrel) and function (NAD(P)H-dependent oxido-reduction of carbonyl groups). Transcripts of this gene have been reported in specimens of human testis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012],
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Western Blot analysis of various cells using AKR1CL2 Polyclonal Antibody diluted at 1:500
Western blot analysis of lysates from HepG2 and Jurkat cells, using AKR1CL2 Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of the lysates from HT-29 cells using AKR1CL2 antibody.
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4 overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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