The antiserum was produced against synthesized peptide derived from human APC. AA range:2794-2843
Alternative Synonym:
APC, DP2.5, Adenomatous polyposis coli protein, Protein APC, Deleted in polyposis 2.5
This gene encodes a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway. It is also involved in other processes including cell migration and adhesion, transcriptional activation, and apoptosis. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product. [provided by RefSeq, Jul 2008],
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Immunofluorescence analysis of Hela cell. 1,APC Polyclonal Antibody(red) was diluted at 1:200(4 overnight). Galectin-3 Monoclonal Antibody(6G2)(green) was diluted at 1:200(4 overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:RS3208 was diluted at 1:1000(room temperature, 50min).
Western Blot analysis of various cells using APC Polyclonal Antibody diluted at 1:500
Western Blot analysis of COLO205 cells using APC Polyclonal Antibody diluted at 1:500
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using APC Antibody. The picture on the right is blocked with the synthesized peptide.
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