ARK-2 rabbit pAb, Rabbit, Polyclonal

Artikelnummer: EBT-ES1701
Artikelname: ARK-2 rabbit pAb, Rabbit, Polyclonal
Artikelnummer: EBT-ES1701
Hersteller Artikelnummer: ES1701
Alternativnummer: EBT-ES1701-50, EBT-ES1701-100
Hersteller: ELK Biotechnology
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, IF, IHC, WB
Spezies Reaktivität: Human, Monkey, Mouse, Rat
Immunogen: The antiserum was produced against synthesized peptide derived from human AurB. AA range:198-247
Alternative Synonym: AURKB, AIK2, AIM1, AIRK2, ARK2, STK1, STK12, STK5, Aurora kinase B, Aurora 1, Aurora- and IPL1-like midbody-associated protein 1, AIM-1, Aurora/IPL1-related kinase 2, ARK-2, Aurora-related kinase 2, STK-1, Serine/threonine-protein kinase 12
This gene encodes a member of the aurora kinase subfamily of serine/threonine kinases. The genes encoding the other two members of this subfamily are located on chromosomes 19 and 20. These kinases participate in the regulation of alignment and segregation of chromosomes during mitosis and meiosis through association with microtubules. A pseudogene of this gene is located on chromosome 8. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2015],
Klonalität: Polyclonal
Konzentration: 1 mg/ml
Molekulargewicht: 39kD
NCBI: 9212
UniProt: Q96GD4
Application Verdünnung: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Western Blot analysis of various cells using ARK-2 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
Immunofluorescence analysis of HeLa cells, using AurB Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from COS7 cells, treated with Nocodazole 1ug/ml 16h, using AurB Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4 overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).