The antiserum was produced against synthesized peptide derived from human BID. AA range:44-93
Alternative Synonym:
BID, BH3-interacting domain death agonist, p22 BID, BID
This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8), CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008],
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Western Blot analysis of various cells using BID Polyclonal Antibody diluted at 1:1000
Western Blot analysis of Jurkat cells using BID Polyclonal Antibody diluted at 1:1000
Immunohistochemistry analysis of paraffin-embedded human brain, using BID Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from Jurkat cells treated with H2O2 100uM 30, using BID Antibody. The lane on the right is blocked with the synthesized peptide.
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