This gene encodes a member of the EF-hand calcium-binding protein family. It is one of three genes which encode an identical calcium binding protein which is one of the four subunits of phosphorylase kinase. Two pseudogenes have been identified on chromosome 7 and X. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009],
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Immunofluorescence analysis of Hela cell. 1,Calmodulin Polyclonal Antibody(green) was diluted at 1:200(4 overnight). (red) was diluted at 1:200(4 overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog:RS3211 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 Catalog:RS3608 was diluted at 1:1000(room temperature, 50min).
Western blot analysis of lysates from 1) HuvEc, 2) HepG2, 3) 293 cells, (Green) primary antibody was diluted at 1:1000, 4over night, secondary antibody(cat:RS23920)was diluted at 1:10000, 37 1hour. (Red) Actin beta Monoclonal Antibody(5B7) (cat:YM3028) antibody was diluted at 1:5000 as loading control, 4 over night,secondary antibody(cat:RS23710)was diluted at 1:10000, 37 1hour.
Western Blot analysis of various cells using Calmodulin Polyclonal Antibody diluted at 1:2000
Western Blot analysis of HuvEc cells using Calmodulin Polyclonal Antibody diluted at 1:2000
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