The antiserum was produced against synthesized peptide derived from human CKI-alpha. AA range:126-175
Alternative Synonym:
CSNK1A1, Casein kinase I isoform alpha, CKI-alpha, CK1
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,function:Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 on Ser-45.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Monomer. Interacts with the Axin complex.,
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Western Blot analysis of various cells using Casein Kinase Ialpha Polyclonal Antibody
Immunofluorescence analysis of HeLa cells, using CKI-alpha Antibody. The picture on the right is blocked with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using CKI-alpha Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from HeLa cells, using CKI-alpha Antibody. The lane on the right is blocked with the synthesized peptide.
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