The antiserum was produced against synthesized peptide derived from human HIF-1alpha. AA range:328-377
Alternative Synonym:
HIF1A, BHLHE78, MOP1, PASD8, Hypoxia-inducible factor 1-alpha, HIF-1-alpha, HIF1-alpha, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, bHLHe78, Member of PAS protein 1, PAS doma
hypoxia inducible factor 1 alpha subunit(HIF1A) Homo sapiens This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2011],
IF: 1:50-200 Western Blot: 1/500 - 1/2000.IP 1:200 Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
Western blot analysis of lysates from 1)Hela cell , 2)Hela cells knockdown by siRNA (F:GCCACAUUCACGUAUAUGATT,R:UCAUAUACGUGAAUGUGGCTT), (Green) primary antibody was diluted at 1:1000, 4over night, Dylight 800 secondary antibody(Immunoway:RS23920)was diluted at 1:10000, 37 1hour. (Red) GAPDH Monoclonal Antibody(5B7) (Immunoway:YM3029) antibody was diluted at 1:5000 as loading control, 4 over night, Dylight 680 secondary antibody(Immunoway:RS23710)was diluted at 1:10000, 37 1hour.
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