Nrf2 rabbit pAb, Rabbit, Polyclonal

Produktübersicht

• Artikelname: Nrf2 rabbit pAb, Rabbit, Polyclonal
• Artikelnummer: EBT-ES2985
• Hersteller Artikelnummer: ES2985
• Alternativnummer: EBT-ES2985-50, EBT-ES2985-100
• Hersteller: ELK Biotechnology
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, IHC, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: The antiserum was produced against synthesized peptide derived from human Nrf2. AA range:556-605
• Alternative Synonym: NFE2L2, NRF2, Nuclear factor erythroid 2-related factor 2, NF-E2-related factor 2, NFE2-related factor 2, HEBP1, Nuclear factor, erythroid derived 2, like 2

Produktbeschreibung

This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters, many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene. [provided by RefSeq, Sep 2015],

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1 mg/ml
• Molekulargewicht: 75-100kD
• NCBI: 4780
• UniProt: Q16236

Anwendungsbeschreibung

• Application Verdünnung: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.

Bilder

Western blot analysis of lysates from 1)Hela cell , 2)Hela cells knockdown by NRF2-siRNA (F:CAUUGAUGUUUCUGAUCUATT,R:UAGAUCAGAAACAUCAAUGTT), (Green) primary antibody was diluted at 1:1000, 4over night, Dylight 800 secondary antibody(Immunoway:RS23920)was diluted at 1:10000, 37 1hour. (Red) GAPDH Monoclonal Antibody(5B7) (Immunoway:YM3029) antibody was diluted at 1:5000 as loading control, 4 over night, Dylight 680 secondary antibody(Immunoway:RS23710)was diluted at 1:10000, 37 1hour.