ACK (phospho Tyr284) rabbit pAb, Rabbit, Polyclonal

Produktübersicht

• Artikelname: ACK (phospho Tyr284) rabbit pAb, Rabbit, Polyclonal
• Artikelnummer: EBT-ES4434
• Hersteller Artikelnummer: ES4434
• Alternativnummer: EBT-ES4434-50, EBT-ES4434-100
• Hersteller: ELK Biotechnology
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, IHC, WB
• Spezies Reaktivität: Human, Mouse
• Immunogen: The antiserum was produced against synthesized peptide derived from human ACK1 around the phosphorylation site of Tyr284. AA range:250-299
• Alternative Synonym: TNK2, ACK1, Activated CDC42 kinase 1, ACK-1, Tyrosine kinase non-receptor protein 2

Produktbeschreibung

This gene encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined. [provided by RefSeq, Jul 2008],

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1 mg/ml
• Molekulargewicht: 120kD
• NCBI: 10188
• UniProt: Q07912

Anwendungsbeschreibung

• Application Verdünnung: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.

Bilder

Western blot analysis of lysates from HepG2 cells, treated with EGF 200ng/ml 30, (Green) primary antibody was diluted at 1:1000, 4over night, secondary antibody(cat:RS23920)was diluted at 1:10000, 37 1hour.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using ACK1 (Phospho-Tyr284) Antibody

Immunofluorescence analysis of A549 cells, using ACK1 (Phospho-Tyr284) Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ACK1 (Phospho-Tyr284) Antibody. The picture on the right is blocked with the phospho peptide.