ACK rabbit pAb, Rabbit, Polyclonal

Produktübersicht

• Artikelname: ACK rabbit pAb, Rabbit, Polyclonal
• Artikelnummer: EBT-ES4435
• Hersteller Artikelnummer: ES4435
• Alternativnummer: EBT-ES4435-50, EBT-ES4435-100
• Hersteller: ELK Biotechnology
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, IHC, WB
• Spezies Reaktivität: Human, Mouse
• Immunogen: The antiserum was produced against synthesized peptide derived from human ACK1. AA range:250-299
• Alternative Synonym: TNK2, ACK1, Activated CDC42 kinase 1, ACK-1, Tyrosine kinase non-receptor protein 2

Produktbeschreibung

This gene encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined. [provided by RefSeq, Jul 2008],

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1 mg/ml
• Molekulargewicht: 115kD
• NCBI: 10188
• UniProt: Q07912

Anwendungsbeschreibung

• Application Verdünnung: Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.

Bilder

Western Blot analysis of A549 cells using ACK Polyclonal Antibody. Secondary antibody(catalog:RS0002) was diluted at 1:20000

Western blot analysis of the lysates from COLO205 cells using ACK1 antibody.

Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4 overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).