The antiserum was produced against synthesized peptide derived from human POLD3. AA range:411-460
Alternative Synonym:
POLD3, KIAA0039, DNA polymerase delta subunit 3, DNA polymerase delta subunit p66
This gene encodes the 66-kDa subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3 to 5 exonuclease activity and plays a critical role in DNA replication and repair. The encoded protein plays a role in regulating the activity of DNA polymerase delta through interactions with other subunits and the processivity cofactor proliferating cell nuclear antigen (PCNA). Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Mar 2012],
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Western Blot analysis of hela cells using DNA pol delta3 Polyclonal Antibody diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
Western blot analysis of lysates from LOVO, HepG2, and mouse brain cells, using POLD3 Antibody. The lane on the right is blocked with the synthesized peptide.
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