NFkappaB-p65 (phospho Thr435) rabbit pAb, Rabbit, Polyclonal

Produktübersicht

• Artikelname: NFkappaB-p65 (phospho Thr435) rabbit pAb, Rabbit, Polyclonal
• Artikelnummer: EBT-ES7023
• Hersteller Artikelnummer: ES7023
• Alternativnummer: EBT-ES7023-50, EBT-ES7023-100
• Hersteller: ELK Biotechnology
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, IHC, IP, WB
• Spezies Reaktivität: Human, Monkey, Mouse, Rat
• Immunogen: The antiserum was produced against synthesized peptide derived from human NF-kappaB p65 around the phosphorylation site of Thr435. AA range:402-451
• Alternative Synonym: RELA, NFKB3, Transcription factor p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3

Produktbeschreibung

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011],

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1 mg/ml
• Molekulargewicht: 60kD
• NCBI: 5970
• UniProt: Q04206

Anwendungsbeschreibung

• Application Verdünnung: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/20000. Not yet tested in other applications.

Bilder

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using NF-kappaB p65 (Phospho-Thr435) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from COS7 cells treated with TNF-alpha, using NF-kappaB p65 (Phospho-Thr435) Antibody. The lane on the right is blocked with the phospho peptide.