CAS Number: 149231-66-3 Molecular Weight: 628.25 Salt Form: N/A Purity: >96% Sequence (3-letter): Ac-Tyr-Val-Ala-Asp-pNA Sequence (1-letter): Ac-YVAD-pNA Storage: -20 ?C or below Solubility: DMSO to 10 mM, soluble in aqueous base (e.g. 0.1 N NH4OH) to 1 mg/mL Caspase 1 (ICE) Substrate (Ac-Tyr-Val-Ala-Asp-pNA) is a substrate for interleukin 1b converting enzyme (ICE) which is a cysteine protease that cleaves specifically at aspartic acid. ICE converts inactive IL-1 beta precursor to a pro-inflammatory cytokine that mediates a wide range of immune and inflammatory response. ICE-related cysteine proteases are required for E1A-induced, p53-dependent apoptosis in baby rat kidney (BRK) cells. Adenovirus E1B 19K protein, which is a potent inhibitor of apoptosis, inhibits activation of these proteases in BRK cells Km = 23 ?M (Casp-1), 29,000 ?m (Casp-3), 874 ?M (Casp-4) Activity is quantified by the release of p-nitroaniline which is measured by absorbance at 405 nm.
