Molecular Weight: 716.75Salt Form: N/APurity: >96%Sequence (3-letter): Ac-Val-Asp-Val-Ala-Asp-AMCSequence (1-letter): Ac-VDVAD-AMCStorage: -20 AC or belowAc-Val-Asp-Val-Ala-Asp-AMC is a fluorogenic substrate for Caspase 2. Caspase 2 part of the ICH-1 sub-family is a cysteine protease with a poorly defined role in apoptosis. It associates with several apoptotic proteins such as death effector filament-forming Ced-4-like apoptosis protein (DEFCAP) RIP-associated Ich-1/Ced-3-homologue protein with a death domain (RAIDD) and apoptosis repressor with caspase recruitment domain (ARC). Caspase 2 can be activated by the PIDDosome formed by the association of PIDD1 and CRADD. Recent work demonstrates that Caspase-2 resides in the mitochondria and is essential for stress-induced apoptosis. Caspase-2 cleaves at an Asp residue with a preferred sequence of Val-Asp-Val-Ala-Asp-X.Activity is quantified by release of free fluorescent 7-amino-4-methylcoumarin (AMC) which excites at 360-380 nm and emits at 440-460 nm.References1) Luca L. Fava Florian J. Bock Stephan Geley Andreas Villunger a€Caspase-2 at a Glancea€ Journal of Cell Science 2012 125: 5911-5915, doi: 10.1242/jcs.115105>2) Lisa Bouchier-Hayes a€The role of caspase-2 in stress-induced apoptosisa€ J Cell Mol Med. 2010 Jun, 14(6a): 1212a€''1224.3) M Lopez-Cruzan R Sharma M Tiwari S Karbach D Holstein C R Martin J D Lechleiter & B Herman a€Caspase-2 resides in the mitochondria and mediates apoptosis directly from the mitochondrial compartmenta€ Cell Death Discovery vol 2 Article number: 16005 (2016).
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