The Collagen Hybridizing Peptides are products of 3-Helix Inc.The collagen hybridizing peptide (CHP) is a novel and unique peptide that specifically binds unfolded collagen chains both in vitro and in vivo [1 2 3]. By sharing the Gly-X-Y repeating sequence of natural collagen CHP has a strong capability to hybridize with denatured collagen chains by reforming the triple helical structure in a fashion similar to DNA fragments annealing to complementary DNA strands. CHP is extremely specific: it has negligible affinity to intact collagen molecules due to lack of binding sites and it is inert towards non-specific binding because of its neutral and hydrophilic nature.CHP is a powerful histopathology tool which enables straightforward detection of inflammation and tissue damage caused by a large variety of diseases as well as tissue remodeling during development and aging [3]. CHP robustly visualizes the pericellular matrix turnover caused by proteolytic migration of cancer cells within 3D collagen culture without the use of synthetic fluorogenic matrices or genetically modified cells [4]. CHP can measure and localize mechanical injury to collagenous tissue at the molecular level [5]. It also enables assessment of collagen denaturation in decellularized extracellular matrix [6]. In addition CHP can be used to specifically visualize collagen bands in SDS-PAGE gels without the need for western blot [7]. B-CHP is labeled with biotin for avidin/streptavidin-mediated detection. B-CHP offers flexible detection options such as non-green fluorescence and HRP-enzyme methods to avoid background and enhance signal.CHP come in three varieties: labeled with biotin (Catalog : BIO) for avidin/streptavidin-mediated including HRP-enzyme methods 5-FAM (Catalog : FLU) for green fluorescence detection and Cy3 (Catalog : RED) for red fluorescence detection.Applications: immunofluorescence immunohistochemistry SDS-PAGE (in-gel Western blot)Shipping Conditions: Express shipping as powder at ambient temperature. Store at -20 AC upon arrival until use.Storage: -20 AC as powder, 4 AC after reconstitution in water no need to aliquot and freeze. Stable for 2 years in solution at 4 AC.Publications Powered by Bioz See more details on Bioz1) a€Targeting and mimicking collagens via triple helical peptide assembliesa€ Curr Opin Chem Biol 2013 17 968-975.2) a€Targeting collagen strand by photo-triggered triple-helix hybridizationa€ Proceedings of the National Academy of Sciences of the United States of America 2012 109 14767-14772. DOI: 10.1073/pnas.1209721109.3) In situ imaging of tissue remodeling with collagen hybridizing peptides. ACS Nano 2017 11 9825. DOI: 10.1021/acsnano.7b031504) Visualizing collagen proteolysis by peptide hybridization: From 3D cell culture to in vivo imaging. Biomaterials 2018 DOI:10.1016/j.biomaterials.2018.08.0395) Molecular level detection and localization of mechanical damage in collagen enabled by collagen hybridizing peptides. Nat. Commun. 2017 8 14913 DOI: 10.1038/ncomms14913.6) Molecular assessment of collagen denaturation in decellularized tissues using the collagen hybridizing peptide. Acta Biomater. 2017. 53 268-278 DOI: 10.1016/j.actbio.2017.01.0797) a€Direct detection of collagenous proteins by fluorescently labeled collagen mimetic peptidesa€ Bioconjugate Chemistry 2013 24 9-16. DOI: 10.1021/bc3005842.8) In tendons differing physiological requirements lead to functionally distinct nanostructures. Sci. Reports 2018. DOI: DOI:10.1038/s41598-018-22741-8Features-More informative reliable and convenient than zymography DQ collagen SHG and TEM-High affinity and unparalleled specificity to collagen with essentially no nonspecific binding-Applicable to all types of collagen from all species relying on collagens secondary structure instead of any defined sequence for binding-Suitable for both frozen and paraffin-embedded sections with no need for antigen retrieval-A non-antibody approach with no species restri
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