The Anti-PEth Autologous IgG ELISA is a 96-well ELISA Assay for Detection of Autologous PEth IgG antibodies. Assay Sensitivity: LOD 80 ng/mL Assay Incubation Time: 3.5 hours Sample Type: Serum and plasma samples Assay Range: 80 ng/mL to 45 µg/mL Sample Considerations: Run Samples at 1:125 dilution in provided sample buffer. Serum demonstrates a 25 to 30% stronger signal than plasma. Species of Sample: Human Sample Volume: 10 µL minimum
Product Description
The Human Anti-PEth Autologous IgG ELISA is a direct assay in which the signal is proportional to the amount of autologous anti-PEth IgG contained in the sample. The serum (or plasma) sample is added to a PEth-coated plate for binding. A peroxidase-linked secondary detector and colorimetric substrate are used to detect the amount of anti-PEth antibody bound to the plate. The colorimetric signal is read at absorbance 450 nm and is directly proportional to the amount of autologous anti-PEth IgG in the sample. Ethanol (EtOH) has multiple effects on humans and other animals. Alcohol addiction and organ damage are active areas of research with a need for research reagents and tools.1 Phosphatidylethanol (PEth) is a direct metabolite of EtOH and has been described as a long-lived biomarker for alcohol ingestion.2, 3 It is possible that PEth is involved in the neurological4, protective5, and harmful6 effects of alcohol, but the mechanisms are still being explored. Autologous anti-PEth antibodies are found in lower abundance in serum from heavier drinkers. This inverse relationship between anti-PEth IgG levels and alcohol ingestion was first reported, by Nissinen et. al.7 Evaluation of serum samples of varying consumption habits has demonstrated a high degree of correlation with ingested alcohol. Measuring these autologous anti-PEth IgG levels may serve as an effective biomarker in monitoring alcohol use.
