Cardiolipin (CL) is an important component of the prokaryotic and eukaryotic inner mitochondrial membrane. In humans, CL is made by cardiolipin synthase from phosphatidylglycerol and cytidinediphosphate-diacylglycerol. Monolysocardiolipin (MCL) is an intermediate in CL remodeling. Acetyl transferase tafazzin mutations impairing linoleic acid transfer to MCL are considered the main reason of Barth syndrome, and autoantibodies to MCL have been found in patients with anti-phospholipid syndrome and lupus. Fluorescent lipids have been used in TLC, electrophoresis, microplatefluorescent assays, and sub-cellular localization experiments.
