PI(4)P PIP Beads
Artikelnummer:
ECH-P-B004A
Produktübersicht
Artikelname:
PI(4)P PIP Beads
Artikelnummer:
ECH-P-B004A
Hersteller Artikelnummer:
P-B004A
Alternativnummer:
ECH-P-B004A-100UL, ECH-P-B004A-1ML
Hersteller:
Echelon Biosciences
Kategorie:
Molekularbiologie
Produktbeschreibung
PI(4)P PIP BeadsTM are composed of agarose with 10 nanomoles of bound PI(4)P per mL of beads, enough for several protein binding experiments. Each 1 mL order of PIP Beads includes a small amount of control beads. Larger quantities of Control Beads are also available for purchase. Storage 4 C - do not freeze Applications PIP BeadsTM are designed for use in protein pull-down experiments to identify and characterize phosphoinositide binding proteins. Possible applications include the isolation of PIP-binding proteins present in a cell lysate or in a mixture of in vitro translated peptides, for separation by SDS-PAGE and further analysis. Featured in Publication 1) Casamayor, A. and M. Snyder (2003). Molecular dissection of a yeast septin: distinct domains are required for septin interaction, localization, and function. Mol Cell Biol 23(8): 2762-77. 2) Hu, Y., Z. Liu, et al. (2005). Phosphoinositol lipids bind to phosphatidylinositol 3 (PI3)-kinase enhancer GTPase and mediate its stimulatory effect on PI3-kinase and Akt signalings. Proc Natl Acad Sci U S A 102(46): 16853-8. 3) Brombacher, E., S. Urwyler, et al. (2009). Rab1 guanine nucleotide exchange factor SidM is a major phosphatidylinositol 4-phosphate-binding effector protein of Legionella pneumophila. J Biol Chem 284(8): 4846-56. 4) Galvez-Santisteban, M., Rodriguez-Fraticelli, A. and Martin-Belmonte, F. (2014). Phosphoinositides Coated Beads Binding Assay. Bio-protocol 4(3): e1039. 5) Jiang, K., et al. (2016). PI(4)P Promotes Phosphorylation and Conformational Change of Smoothened through Interaction with Its C-terminal Tail. PLoS Biol 14(2): e1002375. Being bio-reactive blog: Lipid-Ligand interaction tools: lipids are not scary intermediates anymore Documents Technical Data Sheet
Anwendungsbeschreibung
Anwendungsbeschreibung:
Categories: Biochemical Reagents. Related: Beads, Phosphoinositides, PI(4)P. Ship 1 : Shipping Temp. Ship 2: Gel Ice
