PI(4,5)P2 PIP Beads
Artikelnummer:
ECH-P-B045A
Produktübersicht
Artikelname:
PI(4,5)P2 PIP Beads
Artikelnummer:
ECH-P-B045A
Hersteller Artikelnummer:
P-B045A
Alternativnummer:
ECH-P-B045A-100UL,ECH-P-B045A-1ML
Hersteller:
Echelon Biosciences
Kategorie:
Molekularbiologie
Produktbeschreibung
PI(4,5)P2 PIP BeadsTM are composed of agarose with 10 nanomoles of bound PI(4,5)P2 per mL of beads, enough for several protein binding experiments. Each 1 mL order of PIP Beads includes a small amount of control beads. Larger quantities of Control Beads are also available for purchase. Storage 4 C - do not freeze Applications PIP BeadsTM are designed for use in protein pull-down experiments to identify and characterize phosphoinositide binding proteins. Possible applications include the isolation of PIP-binding proteins present in a cell lysate or in a mixture of in vitro translated peptides, for separation by SDS-PAGE and further analysis. Featured in References 1) Gupta, S., J. C. Fanzo, et al. (2003). T cell receptor engagement leads to the recruitment of IBP, a novel guanine nucleotide exchange factor, to the immunological synapse. J Biol Chem 278(44): 43541-9. 2) Kawahara, T. and J. D. Lambeth (2008). Phosphatidylinositol (4,5)-bisphosphate modulates Nox5 localization via an N-terminal polybasic region. Mol Biol Cell 19(10): 4020-31. 3) Kim, S. K., H. Kim, et al. (2011). Phosphatidylinositol phosphates directly bind to neurofilament light chain (NF-L) for the regulation of NF-L self assembly. Exp Mol Med 43(3): 153-60. 4) Lewis, A. E., et al. (2011). Identification of nuclear phosphatidylinositol 4,5-bisphosphate-interacting proteins by neomycin extraction. Mol Cell Proteomics 10(2): M110 003376. 5) Ulicna, L., et al. (2018). PIP2 epigenetically represses rRNA genes transcription interacting with PHF8. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids 1863(3): 266-275. 6) Huang, D., et al. (2019). Hypoxia induces actin cytoskeleton remodeling by regulating the binding of CAPZA1 to F-actin via PIP2 to drive EMT in hepatocellular carcinoma. Cancer Letters 448(28): 117-127. Being bio-reactive blog: Lipid-Ligand interaction tools: lipids are not scary intermediates anymore Documents Technical Data Sheet
Anwendungsbeschreibung
Anwendungsbeschreibung:
Categories: Biochemical Reagents. Related: Beads, Phosphoinositides, PI(4\,5)P2. Ship 1 : Shipping Temp. Ship 2: Gel Ice
