Purified Anti-PtdIns(3,4,5)P3 monoclonal antibody. Subclass: IgG1 Source: Mouse Applications: ELISA, Alphascreen, Fluorescence Polarization, Protein-Lipid Overlay (PIP Strip)
Product Background: Phosphoinositides (PIPns) are minor components of cellular membranes but are integral signaling molecules for cellular communication. Phosphatidylinositol 3,4,5-trisphosphate (PIP3), formed from PI(4,5)P2 though phosphorylation by PI 3-kinase, activates numerous signaling pathways resulting in cell proliferation, growth, survival, glucose transport and protein synthesis. High PIP3 levels from disregulation of PI3-K have been demonstrated in cancer and inflammatory diseases. PIP3 is hydrolyzed by the phosphatases PTEN to PI(4,5)P2 and SHIP to PI(3,4)P2. Storage 2-8 C for up to 30 days, -20 C for greater than 30 days. Do not freeze thaw multiple times. Featured in Publications 1) Sweeney, G., R. R. Garg, et al. (2004). Intracellular delivery of phosphatidylinositol (3,4,5)-trisphosphate causes incorporation of glucose transporter 4 into the plasma membrane of muscle and fat cells without increasing glucose uptake. J Biol Chem 279(31): 32233-42. 2) Papakonstanti, E. A., A. J. Ridley, et al. (2007). The p110delta isoform of PI 3-kinase negatively controls RhoA and PTEN. Embo J 26(13): 3050-61. 3) Maffucci, T., C. Raimondi, et al. (2009). A phosphoinositide 3-kinase/phospholipase Cgamma1 pathway regulates fibroblast growth factor-induced capillary tube formation. PLoS One 4(12): e8285. 4) Wang, Y., L. M. Weiss, et al. (2010). Coordinate control of host centrosome position, organelle distribution, and migratory response by Toxoplasma gondii via host mTORC2. J Biol Chem 285(20): 15611-8. Product keywords: PIP3, PI(3,4,5)P3, PtdIns(3,4,5)P3, IgG, anti-PIP3
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