Rho A (11B6) Rabbit Monoclonal Antibody, Unconjugated
Artikelnummer:
EKL-AMRE17118
Produktübersicht
Artikelname:
Rho A (11B6) Rabbit Monoclonal Antibody, Unconjugated
Artikelnummer:
EKL-AMRE17118
Hersteller Artikelnummer:
AMRe17118
Alternativnummer:
EKL-AMRE17118-50UL, EKL-AMRE17118-100UL, EKL-AMRE17118-200UL
Hersteller:
EnkiLife
Wirt:
Rabbit
Kategorie:
Antikörper
Applikation:
FC, ICC, WB
Spezies Reaktivität:
Human, Mouse, Rat
Konjugation:
Unconjugated
Alternative Synonym:
RHOA, ARH12, ARHA, RHO12, RHOH12,
Produktbeschreibung
Rho A is a small G protein of the Rho family. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. Mainly associated with cytoskeleton organization, in active state binds to a variety of effector proteins to regulate cellular responses such as cytoskeletal dynamics, cell migration and cell cycle. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers (PubMed:8910519, PubMed: 9121475, PubMed: 31570889). Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis (PubMed: 16236794, PubMed: 12900402). Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion (PubMed: 20974804, PubMed: 23940119). Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly (PubMed: 19934221). The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2- dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization (PubMed: 20937854). Regulates KCNA2 potassium channel activity by reducing its location at the cell surface in response to CHRM1 activation, promotes KCNA2 endocytosis (PubMed: 9635436, PubMed: 19403695). Acts as an allosteric activator of guanine nucleotide exchange factor ECT2 by binding in its activated GTP-bound form to the PH domain of ECT2 which stimulates the release of PH inhibition and promotes the binding of substrate RHOA to the ECT2 catalytic center (PubMed: 31888991). May be an activator of PLCE1 (PubMed: 16103226). In neurons, involved in the inhibiton of the initial spine growth. Upon activation by CaMKII, modulates dendritic spine structural plasticity by relaying CaMKII transient activation to synapse-specific, long-term signaling (By similarity). Acts as a regulator of platelet alpha-granule release during activation and aggregation of platelets (By similarity).
Produkteigenschaften
Klonalität:
Monoclonal
Molekulargewicht:
22kDa
NCBI:
387
UniProt:
P61586
Puffer:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% New type preservative N and 50% glycerol. Store at +4C short term. Store at -20C long term. Avoid freeze / thaw cycle.
Reinheit:
Affinity purification
Formulierung:
Liquid
Target-Kategorie:
RHOA
Anwendungsbeschreibung
Application Verdünnung:
WB 1:1000-1:5000,ICC/IF 1:100-1:200,FC 1:100-1:200
