Human IP-10/CXCL10 (Interferon Gamma Induced Protein 10kDa) ELISA Kit
Artikelnummer:
EKL-EH10232
Hersteller Artikelnummer:
EH10232
Alternativnummer:
EKL-EH10232-96T
Hersteller:
EnkiLife
Kategorie:
Kits/Assays
Spezies Reaktivität:
Human
Alternative Synonym:
C7,IFI10,INP10,SCYB10,crg-2,gIP-10,mob-1,
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IP-10/CXCL10. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IP-10/CXCL10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IP-10/CXCL10, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The OD value is proportional to the concentration of Human IP-10/CXCL10. You can calculate the concentration of Human IP-10/CXCL10 in the samples by comparing the OD of the samples to the standard curve.
