Human SIGLEC8 (Sialic Acid Binding Ig Like Lectin 8) ELISA Kit

Artikelnummer: EKL-EH24777
Artikelname: Human SIGLEC8 (Sialic Acid Binding Ig Like Lectin 8) ELISA Kit
Artikelnummer: EKL-EH24777
Hersteller Artikelnummer: EH24777
Alternativnummer: EKL-EH24777-96T
Hersteller: EnkiLife
Kategorie: Kits/Assays
Spezies Reaktivität: Human
Alternative Synonym: SAF2, SIGLEC-8, SIGLEC8L, Sialoadhesin family member 2
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SIGLEC8. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SIGLEC8 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SIGLEC8, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The OD value is proportional to the concentration of Human SIGLEC8. You can calculate the concentration of Human SIGLEC8 in the samples by comparing the OD of the samples to the standard curve.
Detektionsbereich: 0.15-10ng/mL
Sensitivitaet: 0.07ng/mL
Proben: Serum,plasma and other biological fluids
Target-Kategorie: SIGLEC8