Human ECE2 (Endothelin Converting Enzyme 2) ELISA Kit
Artikelnummer:
EKL-EH25343
Hersteller Artikelnummer:
EH25343
Alternativnummer:
EKL-EH25343-96T
Hersteller:
EnkiLife
Kategorie:
Kits/Assays
Spezies Reaktivität:
Human
Alternative Synonym:
Methyltransferase-like region
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human ECE2. During the reaction, Human ECE2 in the sample or standard competes with a fixed amount of Human ECE2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human ECE2. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of Human ECE2 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Detektionsbereich:
123.46-10000pg/mL
Sensitivitaet:
45.9pg/mL
Proben:
Serum,plasma and other biological fluids
Target-Kategorie:
ECE2
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