Human BLVRB (Biliverdin Reductase B) ELISA Kit

Artikelnummer: EKL-EH26310
Artikelname: Human BLVRB (Biliverdin Reductase B) ELISA Kit
Artikelnummer: EKL-EH26310
Hersteller Artikelnummer: EH26310
Alternativnummer: EKL-EH26310-96T
Hersteller: EnkiLife
Kategorie: Kits/Assays
Spezies Reaktivität: Human
Alternative Synonym: FLR, SDR43U1, Flavin Reductase,NADPH, Short Chain Dehydrogenase/Reductase Family 43U,Member 1, Biliverdin-IX beta-reductase, Green heme-binding protein
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human BLVRB. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BLVRB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BLVRB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The OD value is proportional to the concentration of Human BLVRB. You can calculate the concentration of Human BLVRB in the samples by comparing the OD of the samples to the standard curve.
Detektionsbereich: 0.31-20ng/mL
Sensitivitaet: 0.127ng/mL
Proben: Serum,plasma and other biological fluids
Target-Kategorie: BLVRB