Mouse EAAT2 (Excitatory Amino Acid Transporter 2) ELISA Kit

Artikelnummer: EKL-EM22923
Artikelname: Mouse EAAT2 (Excitatory Amino Acid Transporter 2) ELISA Kit
Artikelnummer: EKL-EM22923
Hersteller Artikelnummer: EM22923
Alternativnummer: EKL-EM22923-96T
Hersteller: EnkiLife
Kategorie: Kits/Assays
Spezies Reaktivität: Mouse
Alternative Synonym: SLC1A2, EAAT2, GLT-1, Solute Carrier Family 1 Member 2, Glutamate/aspartate transporter II, Sodium-dependent glutamate/aspartate transporter 2
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse EAAT2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse EAAT2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse EAAT2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The OD value is proportional to the concentration of Mouse EAAT2. You can calculate the concentration of Mouse EAAT2 in the samples by comparing the OD of the samples to the standard curve.
Detektionsbereich: 0.15-10ng/mL
Sensitivitaet: 0.065ng/mL
Proben: Serum,plasma and other biological fluids
Target-Kategorie: EAAT2