Mouse HADHb (Hydroxyacyl Coenzyme A Dehydrogenase Beta) ELISA Kit
Artikelnummer:
EKL-EM23205
Hersteller Artikelnummer:
EM23205
Alternativnummer:
EKL-EM23205-96T
Hersteller:
EnkiLife
Kategorie:
Kits/Assays
Spezies Reaktivität:
Mouse
Alternative Synonym:
MTPB, Mitochondrial Trifunctional Protein,Beta Subunit, 3-Ketoacyl-Coenzyme A Thiolase/Enoyl-Coenzyme A Hydratase(Trifunctional Protein)Beta Subunit, Beta-ketothiolase
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse HADHb. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse HADHb and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse HADHb, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The OD value is proportional to the concentration of Mouse HADHb. You can calculate the concentration of Mouse HADHb in the samples by comparing the OD of the samples to the standard curve.
Detektionsbereich:
1.56-100ng/mL
Sensitivitaet:
0.61ng/mL
Proben:
Serum,plasma and other biological fluids
Target-Kategorie:
HADHb
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