Porcine TIMP2 (Tissue Inhibitors Of Metalloproteinase 2) ELISA Kit

Artikelnummer: EKL-EP22520
Artikelname: Porcine TIMP2 (Tissue Inhibitors Of Metalloproteinase 2) ELISA Kit
Artikelnummer: EKL-EP22520
Hersteller Artikelnummer: EP22520
Alternativnummer: EKL-EP22520-96T
Hersteller: EnkiLife
Kategorie: Kits/Assays
Spezies Reaktivität: Porcine
Alternative Synonym: CSC-21K, TIMP Metallopeptidase Inhibitor 2
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Porcine TIMP2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Porcine TIMP2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Porcine TIMP2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The OD value is proportional to the concentration of Porcine TIMP2. You can calculate the concentration of Porcine TIMP2 in the samples by comparing the OD of the samples to the standard curve.
Detektionsbereich: 0.15-10ng/mL
Sensitivitaet: 0.062ng/mL
Proben: Serum,plasma and other biological fluids
Target-Kategorie: TIMP2