Rat PPP1R15A (Protein Phosphatase 1, Regulatory Subunit 15A) ELISA Kit

Artikelnummer: EKL-ER22105
Artikelname: Rat PPP1R15A (Protein Phosphatase 1, Regulatory Subunit 15A) ELISA Kit
Artikelnummer: EKL-ER22105
Hersteller Artikelnummer: ER22105
Alternativnummer: EKL-ER22105-96T
Hersteller: EnkiLife
Kategorie: Kits/Assays
Spezies Reaktivität: Rat
Alternative Synonym: PPP1-R15A, PPP1R15-A, PPP1-R15-A, GADD34, Growth Arrest And DNA-Damage-Inducible 34, Myeloid differentiation primary response protein MyD116 homolog
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat PPP1R15A. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PPP1R15A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PPP1R15A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The OD value is proportional to the concentration of Rat PPP1R15A. You can calculate the concentration of Rat PPP1R15A in the samples by comparing the OD of the samples to the standard curve.
Detektionsbereich: 0.31-20ng/mL
Sensitivitaet: 0.118ng/mL
Proben: Serum,plasma and other biological fluids
Target-Kategorie: PPP1R15A